We also created a compressible device for discontinuous leak-free gas-phase incubations, allowing to measure T. This 400 mL container, able to maintain corrosive fluids at hydrostatic (up to 120 MPa) and gas (up to 40 MPa) pressures, at up to 150 ☌, served to assess H2 production of our strain at high gas pressure. Our HP bioreactor for continuous culture underwent major improvements. Methods for HP culture were optimized for H2 studies. barophilus MPT, growing optimally at 40 MPa, was chosen as a model and its metabolism was characterized in an applied context. After having screened isolates for assets in substrate degradation and H2 yields, T. High pressure (HP) could influence the associated metabolism, but was not practically considered. H2, a promising energetic vector, can be synthesized by Thermococcales. furiosus DSM 3638 and provide additional clues for comprehensive modeling of transcriptional regulatory networks in the Archaea cellular domain. These analyses increase our knowledge about gene regulation in P. We also identified seven regulons, which represent 13.5% (279 genes) of the total genes in this archaeon. The most abundant DNA-binding domain corresponds to the winged helix-turn-helix domain with few alternative DNA-binding domains. In structural terms, 45% of these proteins are composed of one structural domain, 41% have two domains, and 14% have three structural domains. In this context, we identified a repertoire of 86 DNA-binding transcription factors (TFs) in the archaeon Pyrococcus furiosus DSM 3638, that are clustered into 32 evolutionary families. However, studies about regulatory mechanisms in this cellular domain are still limited. In recent years, there has been a large increase in the amount of experimental evidence for diverse archaeal organisms, and these findings allow for a comprehensive analysis of archaeal genetic organization.
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